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RNA, U Transporter 1, aa1-360, Recombinant, Human (RNUT1, KPNBL, Snurportin 1, Snurportin-1)

Cat no: R2055-01A

RNA, U Transporter 1, aa1-360, Recombinant, Human (RNUT1, KPNBL, Snurportin 1, Snurportin-1)

SNUPN, also known as KPNBL or RNUT1, is a nuclear import adaptor protein belonging to the Snurportin family. Localizing to the cytoplasm and nucleus, Snurportin-1 contains an N-terminal IBB domain and a trimethylguanosine (m3G)-cap binding domain. It specifically binds the terminal 2,2,7-m3G-cap at the 5' end of U snRNPs and functions to transport U snRNPs into the nucleus via an association with Importin b.\n\nSource:\nRecombinant corresponding to aa1-360 of human Snurportin-1 protein, fused to His-tag at N-terminus, expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MEELSQALAS SFSVSQDLNS TAAPHPRLSQ YKSKYSSLEQ SERRRRLLEL QKSKRLDYVN HARRLAEDDW\nTGMESEEENK KDDEEMDIDT VKKLPKHYAN QLMLSEWLID VPSDLGQEWI VVVCPVGKRA LIVASRGSTS AYTKSGYCVN RFSSLLPGGN \nRRNSTAKDYT ILDCIYNEVN QTYYVLDVMC WRGHPFYDCQ TDFRFYWMHS KLPEEEGLGE KTKLNPFKFV GLKNFPCTPE SLCDVLSMDF\nPFEVDGLLFY HKQTHYSPGS TPLVGWLRPY MVSDVLGVAV PAGPLTTKPD YAGHQLQQIM EHKKSQKEGM KEKLTHKASE NGHYELEHLS \nTPKLKGSSHS PDHPGCLMEN\n\nMolecular Weight: 43.3kD (380aa) confirmed by MALDI-TOF\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R2055-01A

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol, 0.1M sodium chloride.

Purity

~90% (SDS-PAGE)

References

1. Huber J., et al. (1998) EMBO J. 17(14):4114-26.

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