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RNASE7, Recombinant, Human, aa29-156, His-Tag (Ribonuclease RNase A Family 7, MGC133220)

Cat no: 137187

RNASE7, Recombinant, Human, aa29-156, His-Tag (Ribonuclease RNase A Family 7, MGC133220)

RNASE7 is one of the final RNase A superfamily ribonucleases. It was isolated from skin-derived stratum corneum. This protein exhibited potent ribonuclease activity and thus may contribute to the well known ribonuclease activity of human skin. It revealed broad spectrum antimicrobial activity against many pathogenic microorganisms and remarkably potent activity against a vancomycin-resistant Enterococcus faecium. \n\nSource: \nRecombinant protein corresponding to human RNASE7, fused to His-tag at N-terminal expressed in E.coli.\n\nMolecular Weight: \n~16.9kD (151aa) (MALDI-TOF) \n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSKPKGMTS SQWFKIQHMQ PSPQACNSAM KNINKHTKRC KDLNTFLHEP FSSVAATCQT PKIACKNGDK NCHQSHGPVS LTMCKLTSGK YPNCRYKEKR QNKSYVVACK PPQKKDSQQF HLVPVHLDRV L\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

137187

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol, 1mM DTT.

Purity

~90% (SDS-PAGE)

References

1. Zhang J. et al. (2003) Nucleic Acids Res. 31:602-607. 2. Harder J. et al. (2002) J Biol Chem. 277:46779-46784.

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