Rnasen, ID (Drosha, Etohi2, Rn3, Rnasen, Ribonuclease 3, Protein Drosha, Ribonuclease III) (Biotin)

Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies (By similarity).\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

041101-Biotin

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Biotin.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Chong, M.M., et al. Genes Dev. 24(17):1951-1960(2010)\nYang, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(34):15163-15168(2010)\nMichon, F., et al. Dev. Biol. 340(2):355-368(2010)\nWu, H., et al. PLoS ONE 4 (10), E7566 (2009) :\nShenoy, A., et al. PLoS ONE 4 (9), E6971 (2009) :

Additional Info

Human

Alternative Names

Drosha, Etohi2, Rn3, Rnasen, Ribonuclease 3, Protein Drosha, Ribonuclease III