RNF128 (E3 Ubiquitin-protein Ligase RNF128, Gene Related to Anergy in Lymphocytes Protein, RING Finger Protein 128, GRAIL)

GRAIL is a type I transmembrane protein that localizes to the endocytic pathway and contains a PA (protease associated) domain and a RING-type zinc finger domain. It is a ubiquitin-protein isopeptide ligase (E3) necessary for the induction of CD4(+) T cell anergy in vivo and induced expression of this protein was observed in anergic CD4 (+) T cells, which suggested a role in the induction of anergic phenotype. It is differentially expressed in naturally occurring and peripherally induced CD25 (+) T regulatory cells and the expression is linked to the conversion of these cells to a regulatory phenotype. Expression of GRAIL in retrovirally transduced T cell hybridomas limits activation-induced IL-2 and IL-4 cytokine production. It also functions in the patterning of the dorsal ectoderm and sensitizes ectoderm to respond to neural-inducing signals.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nImmunohistochemistry (paraffin): 5-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman, mouse and rat spleen lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R2031-23P

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can, NHP

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Anandasabapathy, N. et al. Immunity. 18:535-547 (2003). 2. Kostianovsky, AM. et al. J. Immunol. 178:6158-6163 (2007). 3. Mackenzie, DA. et al. J. Biol. Chem. 282:9696-9702 (2007).

Additional Info

Recognizes human RNF128. Species Crossreactivity: chimpanzee, canine, equine, monkey, mouse, rat. Species sequence homology: chicken, opossum.