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RNF181, Recombinant, Human, aa1-153, His-Tag (E3 Ubiquitin-protein Ligase RNF181, HSPC238)

Cat no: 138729

RNF181, Recombinant, Human, aa1-153, His-Tag (E3 Ubiquitin-protein Ligase RNF181, HSPC238)

E3 ubiquitin-protein ligase RNF181, also known as RNF181, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. \n\nSource:\nRecombinant protein corresponding to aa1-153 from human RNF181 at N-terminal, fused to His-tag expressed in E.coli. \n\nMolecular Weight: \n~20.3kD (176aa)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGS\nRGSQAELKCP VCLLEFEEEE TAIEMPCHHL FHSSCILPWL SKTNSCPLCR YELPTDDDTY EEHRRDKARK QQQQHRLENL HGAMYT MASYFDE HDCEPSDPEQ ETRTNMLLEL ARSLFNRMDF EDLGLVVDWD HHLPPPAAKT VVENLPRTVI\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

138729

Size

100ug

Form

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.15M sodium chloride, 1mM DTT, 10% glycerol.

Purity

~95% (SDS-PAGE)

References

1. Brophy T.M., et al. (2008) Biochem. Biophys. Res. Commun. 369:1088-1093 2. Gauci S., et al. (2009) Anal. Chem. 81:4493-4501.

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Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, NHP, Prc, Xen/Amph, ZF/Fish

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

WB

Hosts

Mouse

Reactivities

Hum

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Applications

ICC, WB

Hosts

Mouse

Reactivities

Hum

More info
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