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RNF3, 247aa Isoform (Polycomb Group RING Finger Protein 3, RING Finger Protein 3A, PCGF3, RNF3A, MGC40413)

RNF3, 247aa Isoform (Polycomb Group RING Finger Protein 3, RING Finger Protein 3A, PCGF3, RNF3A, MGC40413)

Cat no: R2031-81E

Supplier: United States Biological
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Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2000 Western Blot: 0.5-2ug/ml, human heart lysate observed on ~35kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: R2031-81E
Reactivities: Human, Mouse, Bovine, Canine
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Freemont PS. The RING finger. A novel protein sequence motif related to the zinc finger. Ann N Y Acad Sci. 1993 Jun 11;684:174-92. Review. No abstract available.
Additional info: Recognizes human RNF3. Species sequence homology: Bovine, canine and mouse.

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