RNP AO (Nuclear Ribonucleoprotein, nRNP, hnRNP, Heterogeneous Nuclear Ribonucleoprotein A0)

Heterogeneous nuclear ribonucleoproteins (hnRNP) bind to nascent RNA polymerase II transcripts and are involved in transcript-specific packaging as well as alternative splicing of pre-mRNAs. hnRNPs also appear to influence mRNA metabolism and transport. In vivo, the M proteins bind to mRNA, and in vitro they bind avidly to poly(G) and poly(U) homopolymers. Four different isoforms of hnRNP are produced by alternative splicing: M1, M2, M3, and M4. Ongoing studies have theorized that hnRNPs may cause certain genetic diseases such as myotonic dystrophy, which is the most common form of adult muscular dystrophy. The identification of hnRNPs may therefore be important to determining the cause and cure for genetic diseases.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml detects R2994-01C in RIPA lysates from RAW 264.7 cells. RAW 264.7 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with R2994-01C (0.5ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates hnRNP (38kD).\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies: \nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I\n1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb \nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R2994-01C

Size

200ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Rousseau, S., et al., EMBO J. 21: 6505-6514, 2002.

Additional Info

Recognizes both the phosphorylated and non-phosphorylated forms of human RNP A0, Mr ~38kD. A non-specific protein was also detected, Mr ~35kD. Species Crossreactivity: mouse