RNP (Nuclear Ribonucleoprotein, nRNP, hnRNP)

Heterogeneous nuclear ribonucleoproteins (hnRNP) bind to nascent RNA polymerase II transcripts and are involved in transcript-specific packaging as well as alternative splicing of pre-mRNAs. hnRNPs also appear to influence mRNA metabolism and transport. In vivo, the M proteins bind to mRNA, and in vitro they bind avidly to poly(G) and poly(U) homopolymers. Four different isoforms of hnRNP are produced by alternative splicing: M1, M2, M3, and M4. Ongoing studies have theorized that hnRNPs may cause certain genetic diseases such as myotonic dystrophy, which is the most common form of adult muscular dystrophy. The identification of hnRNPs may therefore be important to determining the cause and cure for genetic diseases.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution: \nELISA: 1:100-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R2995

Size

2ml

Applications

ELISA

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 2ml ddH2O.

P Type

Pab

Purity

Purified by fractionation of delipidated and defibrinated donor human serum.

Isotype

IgG

Additional Info

Recognizes nRNP. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. This product shows no reactivity at a dilution of 1:100 when tested against purified Ro/SS-A, La/SS-B, Sm, Scl-70, Jo-1, Mitochondrial or Ribosomal P antigens in an ELISA system designed to detect IgG antibody.