Ro-52/SS-A Antigen (52kD), Recombinant, Human (SSA1, Sjogren Syndrome Antigen A1, 52kD Ribonucleoprotein Autoantigen SS-A/Ro, RO52, RNF81, Tripartite Motif Protein 21, TRIM21)

TRIM21 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The 52 kDa Ro protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. Ro/SSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Ribonucleoprotein particle is composed of a single polypeptide and one of four small RNA molecules. The RoSSA is present in all mammalian cells studied but has no known function. At least 2 isoforms are present in nucleated and red blood cells, and tissue specific differences in Ro/SSA proteins were identified.\n\nSource:\nHuman, recombinant protein expressed in E. coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 52kD.\n\nProtein Content:\nProtein quantitation was carried out by using a 0.25-2.0mg/ml Bradford assay vs. BSA\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions: \nOptimal dilutions to be determined by the researcher.\n\nAA Sequence:\nMASAARLTMMWEEVTCPICLDPFVEPVSIECGHSFCQECISQVGKGGGSVCAVCRQRFLLKNLRPNRQLANMVNNLKEISQEAREGTQGERCAVHGERLHLFCEKDGKALCWVCAQSRKHRDHAMVPLEEAAQEYQEKLQVALGELRRKQELAEKLEVEIAIKRADWKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCAVHITLDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSGKHYWEVDVTGKEAWDLGVCRDSVRRKGHFLLSSKSGFWTIWLWNKQKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDHGSLIYSFSECAFTGPLRPFFSPGFNDGGKNTAPLTLCPLNIGSQGSTDY\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nAlso Available:\nR2997-21: Ro/SS-A Antigen (52kD), Recombinant, Human\nR2997-22: Ro/SS-A Antigen (60kD), Recombinant, Human\n

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SPECIFICATIONS

Catalog Number

R2997-21

Size

10ug

Form

Supplied as a liquid in 6M Urea, 500mM sodium chloride, 10mM Tris, pH 8.0.

Purity

Chromatographically purified (~90% by RP-HPLC, SDS-PAGE).

References

1. Restricted specificity of intermolecular spreading to endogenous La (SS-B) and 60 kDa Ro (SS-A) in experimental autoimmunity. Gordon TP, Kinoshita G, Keech C, Purcell A, Scand J Immunol 2002 Aug; 56(2): 168-73 2. Comparison of anti 60 and 52 kDa SS-A/Ro antibodies in the pathogenesis of cutaneous lupus erythematosus. Yoshimasu T, Hiroi A, Uede K, J Dermatol Sci 2002 May; 29(1): 35-41 3. Specificity of autoantibodies to SS-A/Ro on a transfected and overexpressed human 60 kDa Ro autoantigen substrate. Fritzler MJ, Hanson C, Eystathioy T, J Clin Lab Anal 2002; 16(2): 103-8 4. Immune responses to SS-A 52-kDa and 60-kDa proteins and to SS-B 50-kDa protein in mothers of infants with neonatal lupus erythematosus. Yukiko N, Br J Dermatol 2000 May 142(5): 908-12 5. 17-beta-estradiol increases expression of 52-kDa and 60-kDa SS-A/Ro autoantigens i n human keratinocytes and breast cancer cell line MCF-7. Wang D, Chan EK, J Invest Dermatol 1996 Oct; 107(4): 610-4 6. Cloning and expression of mouse 60 kDa ribonucleoprotein SS-A/Ro. Wang D, Buyon JP, Mol Biol Rep 1996; 23(3-4): 205-10.

Alternative Names

52kD Ro protein, Sjoegren syndrome type A antigen, SS-A, Ro(SS-A), 52kD ribonucleoprotein autoantigen Ro/SS-A, Tripartite motif-containing protein 21, RING finger protein 81, TRIM21, RNF81, RO52, SSA1, SSA, RO-52