Ro-60/SS-A Antigen (60kD), Recombinant, Human (SSA1, Sjogren Syndrome Antigen A1, 52kD Ribonucleoprotein Autoantigen SS-A/Ro, RO52, RNF81, Tripartite Motif Protein 21, TRIM21)

The SS-A/Ro particle contains a hY RNA (human cytoplasmic RNA) and associated proteins: a 60kD and a 52kD protein. The 52kD protein is not directly bound to the hY RNA but to the 60kD protein. It seems that it is sometimes associated, sometimes not. The Ro antigen is found in the cytoplasm but also in the nucleus. An RNA-binding protein, the Ro 60kD autoantigen, is a major target of the immune response in patients suffering from two systemic rheumatic diseases, systemic lupus erythematosus and Sjogren's syndrome. In lupus patients, anti-Ro antibodies are associated with photosensitive skin lesions and with neonatal lupus, a syndrome in which mothers with anti-Ro antibodies give birth to children with photosensitive skin lesions and a cardiac conduction defect, third degree heart block. In vertebrate cells, the Ro protein binds small RNAs of unknown function known as Y RNAs. Although the cellular function of Ro has long been mysterious, recent studies have implicated Ro in two distinct processes: small RNA quality control and the enhancement of cell survival following exposure to ultraviolet irradiation. Most interestingly, mice lacking the Ro protein develop an autoimmune syndrome that shares some features with systemic lupus erythematosus in patients, suggesting that the normal function of Ro may be important for the prevention of this autoimmune disease.\n\nRecombinant Human SS-A/RO-60 is a single, non-glycosylated, polypeptide chain having a molecular mass of 60kD.\n\nProtein Content:\nProtein quantitation was carried out by using a 0.25-2.0mg/ml Bradford assay vs. BSA\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nAlso Available:\nR2997-21: Ro/SS-A Antigen (52kD), Recombinant, Human\n

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SPECIFICATIONS

Catalog Number

R2997-22

Size

10ug

Form

Supplied as a liquid in 6M Urea, 500mM sodium chloride, 10mM Tris, pH 8.0.

Purity

Chromatographically purified ((same/more than) 90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain). Endotoxin: < 0.1ng/ug (IEU/ug)

References

1. Restricted specificity of intermolecular spreading to endogenous La (SS-B) and 60 kDa Ro (SS-A) in experimental autoimmunity. Gordon TP, Kinoshita G, Keech C, Purcell A, Scand J Immunol 2002 Aug; 56(2): 168-73 2. Comparison of anti 60 and 52 kDa SS-A/Ro antibodies in the pathogenesis of cutaneous lupus erythematosus. Yoshimasu T, Hiroi A, Uede K, J Dermatol Sci 2002 May; 29(1): 35-41 3. Specificity of autoantibodies to SS-A/Ro on a transfected and overexpressed human 60 kDa Ro autoantigen substrate. Fritzler MJ, Hanson C, Eystathioy T, J Clin Lab Anal 2002; 16(2): 103-8 4. Immune responses to SS-A 52-kDa and 60-kDa proteins and to SS-B 50-kDa protein in mothers of infants with neonatal lupus erythematosus. Yukiko N, Br J Dermatol 2000 May 142(5): 908-12 5. 17-beta-estradiol increases expression of 52-kDa and 60-kDa SS-A/Ro autoantigens i n human keratinocytes and breast cancer cell line MCF-7. Wang D, Chan EK, J Invest Dermatol 1996 Oct; 107(4): 610-4 6. Cloning and expression of mouse 60 kDa ribonucleoprotein SS-A/Ro. Wang D, Buyon JP, Mol Biol Rep 1996; 23(3-4): 205-10.