Ro/SS-A Antigen (SSA1, Sjogren Syndrome Antigen A1, 52kD Ribonucleoprotein Autoantigen SS-A/Ro, RO52, RNF81, Tripartite Motif Protein 21, TRIM21)

The (Ro) SS-A antigen is comprised or an acidic 60kD protein that may also be associated with a RNA ranging in size from 80 to 112 bases. The antigenic activity of the (Ro) SS-A antigen appears to be independent of the RNA since the precipitin activity remains after treatment with RNase or separation from the RNA. Anti-(Ro) SS-A autoantibodies were described originally as precipitating autoantibodies in sera of Sjogren's Syndrome and Systemic Lupus Erythematosus patients. The presence of autoantibodies against the (Ro)SS-A antigen may be used as a diagnostic aid. Elevated levels of Anti-(Ro) SS-A have been detected in as high as 96% of Sjogren's Syndrome patients, 49% of patients with Systemic Lupus Erythematosus, 83% of mothers of infants with isolated complete congenital heartblock or Neonatal Lupus Dermatitis, and over 75% of patients with Subacute Cutaneous Lupus. Recently, a strong correlation between low positive levels of Anti-(Ro) SS-A and subdinical dry eyes or mouth was found in a substantial portion of an elderly population. In addition, it has been shown that 70% of patients with (Ro) SS-A precipitating antibodies also have rheumatoid factor. The titer of rheumatoid factor and the concentration of Anti-(Ro) SSA have been closely correlated in Primary Sjogren's Syndrome. Recent developments in ELISA methodology have increased the sensitivity and the ability of the diagnostician to quantitate patient antibody titers.\n\nSource: \nSS-A (RO) Antigen, from calf thymus.\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

R2997-04B

Size

200ug

Applications

ELISA

Form

Supplied as a liquid in 20mM HEPES-NaOH, pH 7.5, 0.32M sodium chloride, 2mM MgCl2, 0.2mM DTT, 0.1mM PMSF, 0.1% preservative, bromo-nitro-dioxane/methylisothiazoline, 20% glycerol.

Purity

(same/more than)90% pure (SDS-PAGE)