ROBO4 Fc Chimera, Recombinant Human (Roundabout Homolog 4, Roundabout Homolog 4 Magic Roundabout, Roundabout Homolog 4 Precursor, FLJ20798, Magic Roundabout, MRB, MGC133352, MGC133353)

ROBO4, also called magic roundabout, is a ~150kD glycoprotein belonging to the ROBO\nfamily (1). ROBOs are molecular guidance receptors that typically interact with Slit ligands to regulate axon guidance and neuronal migration (2). Unlike other family members, ROBO4 is mainly restricted to the vascular endothelium (1, 2). Expression in early hematopoietic progenitors is also reported (3). The human ROBO4 cDNA encodes 1012 amino acids (aa), including a 27 aa signal sequence, a 440 aa extracellular domain (ECD) containing two C2type\nIg domains and two fibronectin type III (FNIII) domains, a transmembrane domain and an intracellular domain. ROBO4 diverges from other ROBOs in the number of Ig, FNIII and cytoplasmic CC domains (1, 4). Within the ECD, human ROBO4 shares 80%, 80%, 87% and 88% aa identity with mouse, rat, bovine and canine ROBO4, respectively. Vascular endothelial ROBO4 is expressed at highest levels in during development and vascular remodeling, including tumor angiogenesis (1, 2, 4 6). It is proposed to contribute to vascular stability. Consistent with this, endogenous ROBO4 is concentrated in the vascular stalk and sprouts rather than tip cells and appears to protect newly formed blood vessels against VEGFinduced vascular leak (6 9).\nROBO4 binding of Slit proteins has been variably reported, and when detected may be mediated by ROBO4/ROBO1 heterodimers (2, 4 7, 9 13). ROBO4 is also variably reported to stimulate or inhibit cell migration or filopodia formation (2, 4 13). Effects on cell movement may be mediated through intracellular binding of WASP, Ras/Rac/Rho, Mena, Srcor Paxillinrelated\nproteins, all of which affect the cytoskeleton (5 7, 10 12). Recombinant soluble ROBO4 ECD can antagonize endothelial cell migration and in vivo angiogenesis (13).\n\nSource: \nRecombinant corresponding to human ROBO4 expressed in NS0 cells.\n\nMolecular Weight: \n~74kD\n\nBiological Activity:\nMeasured by its binding ability in a functional ELISA. When recombinant human ROBO4 Fc Chimera is immobilized at 5ug/ml, biotinylated recombinant rat UNC5H2/Fc Chimera binds with an apparent KD < 20nM.\n\nEndotoxin Level:\n<1EU/ug (LAL method)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R9417-72

Size

50ug

Applications

ELISA

Form

Supplied as a lyophilized powder in PBS. Reconstitute at 400ug/ml in PBS.

Purity

~95% (SDS-PAGE)

References

1. Huminiecki, L. et al. (2002) Genomics 79:547. 2. Legg, J.A. et al. (2008) Angiogenesis 11:13.\n3. Shibata, F. et al. (2009) Stem Cells 27:183. 4. Park, K.W. et al. (2003) Dev. Biol. 261:251. 5. Seth, P. et al. (2005) Biochem. Biophys. Res. Commun. 332:533. 6. Jones, C.A. et al. (2008) Nat. Med. 14:448.\n7. Jones, C.A. et al. (2009) Nat. Cell Biol. 11:1325. 8. Chen, H. et al. (2010) Adv. Exp. Med. Biol. 64:457.\n9. London, N.R. et al. (2009) J. Thromb. Haemost. 7:57. 10. Verissimo, A.R. et al. (2009) Biochem. Soc. Trans. 37:1214. 11. Sheldon, H. et al. (2009) FASEB J. 23:513. 12. Kaur, S. et al. (2008) BMC Cell Biol. 9:61. 13. Suchting, S. et al. (2005) FASEB J. 19:121.