ROC1

ROC1 and ROC2 are the two highly conserved RING finger proteins that are homologous to APC11, a subunit of the anaphase-promoting complex (APC). ROC1 and ROC2 commonly interact with all cullins while APC11 specifically interacts with APC2, a cullin-related APC subunit. ROC1 and APC11 immunocomplexes can catalyze isopeptide ligations to form polyubiquitin chains in an E1-and E2-dependent manner. ROC1 is thought to play a unique role in the ubiquitination reaction by heterodimerizing with cullin1 to catalyze ubiquitin polymerization. \n\nEpitope: \naa97-108\n\nApplications:\nSuitable for Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution: \nImmunoprecipitation: 10ug/mg protein lysate (Use Protein A; Native verified)\n\nWestern Blot: 5-10ug/ml for 2hrs at RT \n\nImmunohistochemistry: 10-20ug/ml for 30 min at RT (Formalin/paraffin). No special pretreatment is required for immunohistochemical staining of formalin/paraffin tissues.\n\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHeLa cells and tonsil. \n\nCellular Localization: \nNuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degrees C or colder. Aliquots are stable for at least 12 months at-20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R2997-80

Size

500ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See R2997-80X.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Ohta, T., et al., Mol. Cell 3(4): 535 (1999). 2. Tan, P., et al., Mol. Cell 3(4): 527 (1999). 3. Skowyra, D., et al., Science 284(5414): 662 (1999).

Additional Info

Recognizes human ROC1 at 13kD. Species Crossreactivity: Mouse.