ROC1 (Rbx1, Hrt1, RING Finger Protein)

ROC1, also called Rbx1 or Hrt1, is a RING finger protein that is homologous to APC11, a subunit of the anaphase-promoting complex. ROC1 commonly interacts with all cullins and is one of the subunits of the SCF ubiquitin protein ligases. In yeast, ROC1 encodes an essential gene whose expression results in multiple, elongated buds and accumulation of Sic1p and Cln2p. ROC1 immunocomplexes can catalyze isopeptide ligations to form polyubiquitination chains in an E1- and E2-dependent manner. For example, phosphorylated IkBa ubiquitination can be catalyzed by the ROC1 immunocomplex in vitro. ROC1 mutations completely abolish their ligase activity without noticeable changes in associated proteins.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:750\nImmunoprecipitation:1-2ug antibody per 200-500ug of protein lysate. Note: Reported to co-immunoprecipitate associated cullin and ubiquitin ligase activity.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHeLa, CTLL and NIH-3T3 cells. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

R2997-75

Size

500ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by Protein G chromatography.

Isotype

IgG

References

1. Seol, H-J., et al., 1999, Genes Dev. 13: ,614-1626. 2. Ohta, T., et al., 1999, Mol. Cell 3: 535-541. 3. Tan, P., et al., 1999, Mol. Cell 3: 527-33. 4. Iwai, K., et al., 1999, Nat

Additional Info

Recognizes a protein of 15kD, identified as ROC1. Species Crossreactivity: Human and mouse ROC1 protein.