ROK alpha (Rho-associated Kinase-alpha, ROK-alpha, Rho-associated Coiled-coil Containing Protein Kinase 2, Rho-associated Protein Kinase 2, Rho Kinase 2, ROCK2, Rock2m, ROCK-2, p164 ROCK-2, ROCK-II, Rho-kinase)

The small GTPase Rho is implicated in cytoskeletal rearrangements including stress fiber and focal adhesion formation and in the transcriptional activation of c-fos serum response element. In vitro, Rho-kinase, which is activated by Rho, phosphorylates not only myosin light chain (MLC) (thereby activating myosin ATPase) but also myosin phosphatase, thus inactivating myosin phosphatase. Rho-kinase is involved in the formation of stress fibers and focal adhesions in fibroblasts.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R2998-26A

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

As reported

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Additional Info

Specific for human Rho Kinase alpha protein. Other species have not been tested. Reactivity with mouse Rho Kinase alpha is likely owing to high similarity between this ortholog and human Rho Kinase alpha at the epitope that is recognized by the antibody.