ROK alpha/ROCK-II (Rho-associated protein kinase 1 / Rho-associated coiled-coil containing protein kinase 2)

Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKa/ROCK-II and ROKb/ROCK-I. These large cellular proteins (150-160kD), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.\n\nApplications:\nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:5000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nRIPA lysates from PC-12 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R2998-26C

Size

200ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid, 0.05% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG

Additional Info

Recognizes rat ROKa/ROCK-II, Mr 150kD. Does not recognize ROKb/ROCK-I. A non-specific band may also be detected, Mr 80kD. Species Crossreactivity: human. Species sequence homology: mouse and bovine.