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ROR gamma T (RORC2, NR1F3, RORgammaT) (PE)

Cat no: R1183-30H

ROR gamma T (RORC2, NR1F3, RORgammaT) (PE)

ROR-gamma T is a member of the orphan nuclear receptor family. ROR-gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR-gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5'A/T-rich extensions. An N-terminal isoform of ROR-gamma, RORgammaT, has been shown to be specifically expressed in the thymus. \n\nApplications:\nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 10ul labels (same/less than) 1x10e6 cells. For intracellular staining, cells must first be fixed and permeabilized. 4% PFA is recommended as a fixative and a 0.1% saponin balanced salt solution is recommended for permeabilization and washing.\nOptimal dilutions to be determined by the researcher.\n\nFlow Cytometry Protocol:\n1. Harvest cells and wash twice in saline buffer.\n2. Cell surface staining may be done at this point following the manufacturer

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SPECIFICATIONS

Catalog Number

R1183-30H

Size

100Tests

Applications

FC

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

Additional Info

Recognizes human ROR gamma T. Does not react with RORC. Species Crossreactivity: mouse.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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