ROS1 (Receptor Tyrosine Kinase, V-ros UR2 SArcoma Virus) (Biotin)

ROS1, an orphan receptor tyrosine kinase of the insulin receptor family, was initially identified as a homolog of v-ros from the UR2 sarcoma virus (1). ROS1 consists of a large extracellular domain that is composed of six fibronectin repeats, a transmembrane domain, and an intracellular kinase domain. While the function of ROS1 is undefined, it has been shown to play an important role in differentiation of epididymal epithelium (2). The first oncogenic fusion of ROS1, FIG-ROS1, was initially identified by research studies in glioblastoma (3), and subsequent studies have found this fusion in cholangiocarcinoma (4), ovarian cancer (5) and non-small cell lung cancer (NSCLC) (6). Investigators have found additional oncogenic ROS1 fusion proteins in NSCLC (at a frequency of ~1.6%), where the ROS1 kinase domain is fused to the amino-terminal region of a number of different proteins, including CD74 and SLC34A2 (6-8). ROS1 fusion proteins activate the SHP-2 phosphatase, PI3K/Akt/mTOR, Erk, and Stat3 pathways (3,4,9).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137487

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in PBS, 12 mM sodium phosphate, pH 7.4, 2 mg/ml BSA, and 50% glycerol. Labeled with biotin.

P Type

Mab

Purity

Purified.

Isotype

IgG

References

1. Matsushime, H. et al. (1986) Mol Cell Biol 6, 3000-4. 2. Yeung, C.H. et al. (1999) Biol Reprod 61, 1062-9. 3. Charest, A. et al. (2003) Genes Chromosomes Cancer 37, 58-71. 4. Gu, T.L. et al. (2011) PLoS One 6, e15640. 5. Birch, A.H. et al. (2011) PLoS One 6, e28250. 6. Rimkunas, V.M. et al. (2012) Clin Cancer Res 18, 4449-57. 7. Rikova, K. et al. (2007) Cell 131, 1190-203. 8. Stumpfova, M. and J

Additional Info

Recognizes endogenous levels of total human ROS1. Does not cross-react with other related proteins.