RPA1, CT (RPA1, REPA1, RPA70, Replication protein A 70kD DNA-binding subunit, Replication factor A protein 1, Single-stranded DNA-binding protein) (AP)

RPA1 plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.\n\nApplications:\nSuitable for use in Western Blot, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-500\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

041173-AP

Size

200ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to Alkaline Phosphatase.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Choi, J.H., et al. Proc. Natl. Acad. Sci. U.S.A. 107(31):13660-13665(2010)\nLocatelli, G.A., et al. Biochem. J. 429(3):573-582(2010)\nBriggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)\nGuillem, V.M., et al. Am. J. Hematol. 85(7):482-486(2010)\nOakley, G.G., et al. Front. Biosci. 15, 883-900 (2010) :

Additional Info

Human

Alternative Names

RPA1, REPA1, RPA70, Replication protein A 70kD DNA-binding subunit, Replication factor A protein 1, Single-stranded DNA-binding protein