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RPA2 (Replication Protein A2, 32kD) Recombinant

Cat no: R3402

RPA2 (Replication Protein A2, 32kD) Recombinant

Replicatin Protein A2 (32kD) is absolutely required for Simian Virus 40 DNA replication in vitro. It participates in the very early steps of initiation. RPA is a single-stranded DNA-binding protein. It is a hetrotrimer of 70, 32/30 and 14kD chains. The DNA-binding activity may reside exclusively on the 70kD subunit. It is phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis).\n\nApplications: Suitable for use in ELISA, Western Blot and MS. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months. \n\nImportant note: This recombinant enzyme has NOT been tested with regard to biological activity.

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SPECIFICATIONS

Catalog Number

R3402

Size

50ug

Applications

ELISA

Form

Supplied as a liquid in 10mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% sodium azide.

Purity

Highly purified ((same/more than) 95%)\nQuality Control: SDS-PAGE\nDomain (aa): 270aa, Full-length\nProtein GI Number: 4506585\nProtein Accession Number: NP_002937

References

1. Erdile, L.F., Wold, M.S., Kelly, T.J.: The primary structure of the 32kD subunit of human replication protein A. J. Biol. Chem. 265(6): 3177-3182 (1990).

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