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RPA70 (Replication Protein A 70kD DNA-binding Subunit, RP-A p70, Replication Factor A Protein 1, RF-A Protein 1, Single-stranded DNA-binding Protein, REPA1, RPA1)

Cat no: R3400-01E

RPA70 (Replication Protein A 70kD DNA-binding Subunit, RP-A p70, Replication Factor A Protein 1, RF-A Protein 1, Single-stranded DNA-binding Protein, REPA1, RPA1)

Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB) is involved in DNA replication, repair, and recombination. RPA from human cells is a stable heterotrimer of 70kD, 32-34kD, and 11-14kD subunits (RPA70, RPA32, and RPA14 respectively). RPA is required for the SV40 large tumor antigen- catalyzed unwinding of SV40 DNA and stimulates DNA polymerase alpha and delta. RPA70 is composed of three domains: an N-terminal domain that is not required for ssDNA binding or SV40 replication, a central DNA-binding domain, and a C-terminal domain that is essential for subunit interactions.\n\nApplications:\nSuitable for use in Gel supershift Assay, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml for 2h\nImmunoprecipitation: 2ug/mg protein lysate\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHeLa cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R3400-01E

Size

50ug

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

Additional Info

Recognizes human RPA.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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