rSec6

rSec6 is an 87kD rat homolog of yeast Sec6p, a protein involved in protein transport between the Golgi apparatus and the plasma membrane. In yeast, Sec6p is part of a high molecular weight complex which includes Sec8p and Sec15p, that associates with the ras-like GTP binding protein Sec4p. An analogous 17S complex in rat brain was found to contain eight novel proteins, including rSec6 and rSec8, with a combined molecular weight of 734kD. Membrane-associated rSec6/8 complex has been shown to co-immunoprecipitate with syntaxin, and is ubiquitously expressed, further evidence that this complex is involved in the mammalian exocyst complex. \n \nApplications: \nSuitable for use in ELISA, Immunocytochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot (ECL): 1:250.\nOptimal dilutions to be determined by researcher.\n \nPositive Controls:\nRat Brain Tissue Extract. \nMouse Brain Tissue Extract\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R9356

Size

25ug

Applications

ICC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Can, Ch/Bird, NHP, Prc, Rabbit, Sheep

Form

Supplied as a liquid in PBS, pH7.2, 0.1mM PMSF, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a,k

References

1. Kee, Y., et al., (1997) Proc. Natl. Acad. Sci. USA 94:14438-14443. 2. TerBush, D.R. and Novick, P. (1995) J. Cell Biol. 130:299-312. 3. Hsu, S.-C., et al., (1996) Neuron 17(6):1209-1219. 4. Charron, A.J., et al., (2000) J. Cell Biol. 149(1):111-124. 5. Brymora, A., et al., (2001) J. Biol. Chem. 276(32):29792-29797.

Additional Info

Recognizes rSec6 at 87kD. Species Cross Reactivity: human, mouse, rat, bovine, canine, chicken, hamster, sheep, porcine, monkey and rabbit. Immunofluorescence of PC12 cells shows co-localization of rSec6 with vesicles and sites of exocytosis.