Rsk, 90kD, phosphorylated (Thr359/Ser363) (Ribosomal S6 Kinase, p90RSK)

90 kD ribosomal S6 kinases (RSK1-3) are a family of broadly expressed serine/ threonine kinases activated by extracellular signal-regulated protein kinases (Erk1 and 2) in response to many growth factors, polypeptide hormones and neurotransmitters (1). p90RSK is activated by MAPK in vitro and in vivo via phosphorylation (4). Several sites, such as Ser380, Thr359 and Ser363, are important for its activation (3). RSK3 resembles p90RSK by having two kinase domains connected by a regulator linker region (2). Phosphorylation of p90RSK (and likely RSK2 and RSK3) in the linker region is critical for its activity (3).\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:1000 \nImmunoprecipitation: 1:50\nImmunohistochemistry: (Paraffin) 1:250 \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R9399-01B

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Pab

Purity

Purified by Protein A and protein affinity chromatography.

Isotype

IgG

References

1. Small, G.W., et al., J. Pharmacol. Exp. Ther. 307(3): 861-869 (2003). 2. Wu, J., et al.: J. Biol. Chem. 277: 19498-19505 (2002). 3. Zhang, Y., et al.: J. Biol. Chem. 277: 19042-19048 (2002). 4. Zhu, Y., et al. J. Neurosci. 22: 3898-3909 (2002). 5. Frodin, M., Gammeltoft, S., Mol. Cell. Endocrinol. 151: 65-77 (1999). 6. Pierrat, B., et al., J. Biol. Chem. 273: 29661-29671 (1998). 7. Dalby, K., et al., J. Biol. Chem. 273: 1496-1505 (1998). 8. Lazar, D.F., et al., J. Biol. Chem. 270: 20801-20807 (1995). 9. Confocal images of double immunostaining with R9399-01B (green) and propidium iodide (red) in rat hippocampal CA1 regions, from tissue sections of control (left) and 15 minute transient cerebral ischemia (right) followed by 30 minutes of reperfusion. Yellow represents overlay of red and green. (Provided by Dr. Bingren Hu, University of Miami School of Medicine, Florida).

Additional Info

Recognizes endogenous levels of rat p90RSK only when phosphorylated at Thr359/Ser363. Does not recognize p90RSK phosphorylated at other sites. Some crossreactivity with RSK3 when phosphorylated at the homologous sites. Does not crossreact with phospho-RSK2 or MSK1. Species Crossreactivity: Human, monkey and mouse.