Rsk, 90kD, phosphorylated (Thr573) (Ribosomal S6 Kinase, p90RSK)

The 90kD ribosomal S6 kinases (RSK1-3) are a family of broadly expressed serine/ threonine kinases activated by extracellular signal-regulated protein kinases (Erk1 and 2) in response to many growth factors, polypeptide hormones and neurotransmitters. p90RSK (PSK1) lies immediately downstream of MAP kinase in the phorbol ester and growth factor singaling pathway (1,2). PSK1 is activated by MAPK in vitro and in vivo via phosphorylation (3). Several phosphorylation sites, including Ser380, Thr359 and Ser363, are important for its activation (4,5). RSK3 resembles PSK1 by having two kinase domains connected by a regulator linker region. Phosphorylation of PSK1 (and likely RSK2 and RSK3) in the linker region is critical for its activity. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R9399-01C

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Ch/Bird, Xen/Amph

Form

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Also available without BSA. See R9399-01C1.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Blenis, J., PNAS USA, 90: 5889-5892 (1993). 2. Sturgill, T.W., et al., Nature 334: 715-718 (1988). 3. Lazar, D.F., et al., J. Biol. Chem. 270: 20801-20807 (1995). 4. Dalby,

Additional Info

Detects endogenous human p90RSK protein only when phosphorylated at Thr573. Species Crossreactivity: Hamster, mouse and rat. Predicted to crossreact with chicken and Xenopus based on sequence homology.