RSK1, active, Recombinant, Human (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Pr

N-terminal His6 tagged recombinant full-length human RSK1 expressed in Sf21 cells. Activated\nwith PDK1 and MAP Kinase 2/Erk2 and repurified using glutathione-Sepharose, heparin-Sepharose and Ni2+/NTA agarose. \n\nSpecific Activity: \n~2900U/mg,\n\nUnit Definition:\nOne unit of RSK1 activity is defined as 1nmol phosphate incorporated into 30uM M2355-09 MAPKAP Kinase 2 Substrate Peptide (KKLNRTLSVA) per minute at 30 degrees C with a final ATP concentration of 100uM.\n\nIdentity Confirmation:\nMS Tryptic Fingerprint: Confirmed identity as RSK1 with 38% amino acid coverage of the translated sequence.\n\nApplications:\nKinase Assay: Kinase Assay: 0.3-2.5ng phosphorylated 30uM MAPKAP Kinase 2 Substrate Peptide in the assay. Assay background was subtracted from the actual counts to yield the results\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

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SPECIFICATIONS

Catalog Number

R9399-10

Size

5ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride pH 7.5, 0.03% Brij 35, 0.1mM EGTA, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine and 0.1% 2-mercaptoethanol.

Purity

~94% by SDS-PAGE and Coomassie blue staining.

Alternative Names

Ribosomal Protein S6 Kinase 90kDa Polypeptide 1, EC=2.7.11.1, HU-1