RSK1, phosphorylated (Ser380) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ribosomal Prote

RSK1 (also called MAPK Activated Protein Kinase-1a; MAPKAP-K1a) is a member of the broadly expressed p90 Ribosomal S6 Kinase (RSK) family of serine/threonine kinases that also includes RSK2 (MAPKAP-K1b), RSK3 (MAPKAP-K1c), and RSK4. RSK proteins are substrates for and downstream transducers of MAPK signaling proteins, primarily ERK1&2. RSK proteins possess two separate kinase domains, the C-terminal kinase domain and the N-terminal kinase domain, separated by a linker region containing a hydrophobic motif. Activation of RSK1 is a multi-step process involving phosphorylation of multiple residues within the three domains, changes in RSK protein localization, and modulation of RSK complex formation with ERK1&2 and/or 14-3-3 beta proteins. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock, and irradiation. ERK 1&2 phosphorylates threonine 573 of the C-terminal kinase domain, thereby stimulating this kinase activity and leading to phosphorylation of serine 380 within the linker region. Phosphoserine 380 is part of a docking site for PDK1, a constitutively active cytoplasmic kinase that phosphorylates serine 221 and thereby activates the N-terminal kinase domain to phosphorylate various substrates that include transporters, transcription factors, and transcription co-activators.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: A431 and NIH3T3 cells treated with PMA, a phorbol ester activator of protein kinase C.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R9399-07L

Size

50ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

0.05% Sodium Azide; 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Roux PP et al. Phosphorylation of p90 ribosomal S6 kinase (RSK) regulates extracellular signal-regulated kinase docking and RSK activity. Mol Cell Biol 23:4796-804 (2003). <PUBMED:12832467>Kim KW et al. Extracellular signal-regulated kinase/90-KDA ribosomal S6 kinase/nuclear factor-kappa B pathway mediates phorbol 12-myristate 13-acetate-induced megakaryocytic differentiation of K562 cells. J Biol Chem 276:13186-91 (2001). <PUBMED:11278385>Richards SA et al. Characterization of regulatory events associated with membrane targeting of p90 ribosomal S6 kinase 1. Mol Cell Biol 21:7470-80 (2001). <PUBMED:11585927>Zhang Y et al. UVA induces Ser381 phosphorylation of p90RSK/MAPKAP-K1 via ERK and JNK pathways. J Biol Chem 276:14572-80 (2001). <PUBMED:11278279>

Additional Info

Recognzies human RSK1, phosphorylated at Ser380. Crossreaction with RSK2 (85% homologous) is expected based on peptide competition by the analogous sequence from RSK2. Crossreaction with RSK3 (77% homologous) and RSK4 (85% homologous) is possible but has not been tested. Species Crossreactivity: Crossreacts with Human and Mouse. Expected to crossreact with Rat (100% identity with immunogen) due to sequence homology.

Alternative Names

Ribosomal Protein S6 Kinase 90kDa Polypeptide 1, EC=2.7.11.1, HU-1