RSK1, RSK2, phosphorylated (Ser221/Ser227) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ri

RSK1 (also called MAPK Activated Protein Kinase-1a; MAPKAP-K1a) is a member of the broadly expressed p90 Ribosomal S6 Kinase (RSK) family of serine/threonine kinases that also includes RSK2 (MAPKAP-K1b), RSK3 (MAPKAP-K1c) and RSK4. RSK proteins are substrates for and downstream transducers of, MAPK signaling proteins, primarily ERK1&2. RSK proteins possess two separate kinase domains, the C-terminal kinase domain and the N-terminal kinase domain, separated by a linker region containing a hydrophobic motif. Activation of RSK is a multi-step process involving phosphorylation of multiple residues within the three domains, changes in RSK protein localization and modulation of complex formation with ERK1&2 and/or 14-3-3beta proteins. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock and irradiation. ERK1&2 phosphorylate threonine 573 (threonine 577 in RSK2) of the C-terminal kinase domain, thereby stimulating this kinase activity and leading to phosphorylation of serines 363 and 380 (serines 369 and 386 in RSK2) within the linker region. This phosphorylation is required for activation of the N-terminal kinase domain by PDK1. The activated N-terminal kinase domain phosphorylates various substrates that include transporters, transcription factors and transcription co-activators.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested\n\nRecommended Dilution: \nWestern Blot: 1:1000\nOptimal dilution determined by the researcher. \n\nPositive Controls:\nA431 cells treated with 100ng/mL PMA for 30 minutes at 37C; HEK293E cells transfected with human RSK1, chicken RSK1, mouse RSK2, or human RSK3.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R9399-15

Size

10 Blots

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Ch/Bird

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

References

Cavet, M.E., et al. (2003) J. Biol. Chem. 278(20):18376-18383. Roux, P.P., et al. (2003) Mol. Cell. Biol. 23(14):4796-4804. Kim, K.-W., et al. (2001). J. Biol. Chem. 276(16):13186-13191. Richards, S.A., et al. (2001). Mol. Cell. Biol. 21(21):7470-7480. Zhang, Y., et al. (2001) . J. Biol. Chem. 276(18):14572- 14580. Jensen, C.J., et al. (1999). J. Biol. Chem. 274(38):27168-27176. Smith, J.A., et al. (1999) J. Biol. Chem. 274(5):2893-2898.

Additional Info

Recognizes human Rsk1, Rsk2, phosphorylated, Ser221, Ser227.. Species Crossreactivity: mouse and chicken Rsk1 and mouse Rsk2. Species sequence homology: Mouse and human RSK2 (100%) rat RSK1 (100%) and human RSK4 (90%) Does not cross-react with with human RSK3.

Alternative Names

Ribosomal Protein S6 Kinase 90kDa Polypeptide 1, EC=2.7.11.1, HU-1 / Ribosomal Protein S6 Kinase 90kDa Polypeptide 3, CLS, EC=2.7.11.1, HU-3, MRX19, RSK