RSK2, NT (Ribosomal S6 Kinase 2, RSK-2, p90-RSK2, pp90RSK2, 90kD Ribosomal Protein S6 Kinase 3, p90-RSK 3, p90RSK3, Insulin-stimulated Protein Kinase 1, ISPK1, ISPK-1, MAP Kinase-activated Protein Kinase 1b, MAPK-activated Protein Kinase 1b, MAPKAP Kinase

Members of the RSK (the p90 ribosomal S6 kinase) family of serine/threonine kinases are substrates of ERK (the Ras/extracellular signal-regulated kinase) (1). The RSK family consists of four isoforms (RSK1 to -4) and two structurally related cousins, called RSK-like protein kinase (RLPK/MSK1) and RSKB (MSK2). RSK2 is expressed in most tissues including heart, brain, placenta, liver, kidney and pancreas, with predominant expression in skeletal muscle (4-5). The small death effector domain protein PEA-15 inhibits RSK2 nuclear translocation, but the biological function of this interaction is unknown6. Activated RSK2 can also be found in the cytoplasm of stimulated cells suggesting that RSK2 substrates may exist in both nuclear and cytoplasmic compartments (7). A phosphate-binding pocket in the kinase domain of RSK2 is used for intramolecular interaction with its own phosphorylated hydrophobic motif, an interaction that induces a synergistic stimulation of RSK2 catalytic activity, whereas mutation of the phosphate-binding pocket leads to a reduction in the overall kinase activity of RSK28. Defects in the RSK2 gene are the cause of Coffin-Lowry syndrome, an X-linked dominant disorder characterized by psychomotor and growth retardation and facial, hand and skeletal malformations9-10. Numerous mutations have been identified in the RSK2 gene that result in truncated or inactive RSK2 proteins (11-12).\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-3ug/ml. Identifies a single band at ~84 kD\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R9400-01B

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Frodin M and Gammeltoft S. Mol Cell Endocrinol 151:65-77, 1999. 2. Pearson G, et al. Endocr Rev 22:153-183, 2001. 3. Schaeffer HJ and Weber MJ. Mol Cell Biol 19:2435-2444, 1999. 4. Dufresne SD, et al. Mol Cell Biol 16:1212-1219, 2001. 5. Zeniou M, et al. Hum Mol Genet 11:2929-2940, 2002. 6. Vaidyanathan H and Ramos JW. J Biol Chem 278:32367-32372, 2003. 7. Chen RH, et al. Mol Cell Biol 12:915-927, 1992. 8. Frodin M, et al. Embo J 21:5396-5407, 2002. 9. Jacquot S, et al. Am J Med Genet 85:214-215, 1999. 10. Trivier E, et al. Nature 384:567-570, 1996. 11. Delaunoy J, et al. Hum Mutat 17:103-116, 2001. 12. Jacquot S, et al. Am J Med Genet 63:1631-1640, 1998.

Additional Info

Recognizes human RSK2 at ~84kD. Species crossreactivity: mouse. \nDoes not react with overexpressed RSK1, RSK3 or RSK4. Reactivity has been confirmed with endogenously human placenta homogenates, HEK293 cell lysates, and fetal and adult\nmouse skeletal muscle homogenates, and exogenously with RSK-2-transfected HEK293 cells.

Alternative Names

Ribosomal Protein S6 Kinase 90kDa Polypeptide 3, CLS, EC=2.7.11.1, HU-3, MRX19, RSK