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RSPO-3 (R-Spondin 3, Cristin-1)

Cat no: R9417-50

RSPO-3 (R-Spondin 3, Cristin-1)

RSPO-3 (R-spondin 3; also Cristin-1), is a 256aa secreted R-Spondin family member which binds LRP-6 and Fzd8 and regulates b-catenin signaling. R-Spondins contain two adjacent cysteine-rich furin-like domains at their amino-termini, followed by a thrombospondin (TSP-1) motif and a variable length basic C-terminal region. Two RSPO-3 splice isoforms that differ in their C-termini have been reported. Mouse RSPO-3 shares 93% and 97% aa identity with human and rat RSPO-3, respectively, over the sequence used as an immunogen.\n\nApplications: \nSuitable for use in Direct ELISA and Western Blot. Other applications have not been tested.\n\nRecommended Dilution: \nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by researcher.\n\nHybridoma: \nProduced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant mouse RSPO-3.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R9417-50

Size

100ug

Applications

ELISA, WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS, pH 7.4.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes mouse RSPO-3. No crossreactivity with recombinant mouse RSPO-1, RSPO-4, recombinant human RSPO-2 or RSPO-3.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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