RSPO1 (R-spondin-1, Roof Plate-specific Spondin-1, hRspo1)

RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular b-catenin, thereby regulating functions mediated by b-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels. At least two isoforms of RSPO1 are known to exist. RSPO1 antibody will not cross-react with RSPO family members.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml\nImmunofluorescence: 20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRat Spleen Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146444

Size

100ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kamata T, Katsube K, Michikawa M, et al. R-spondin, a novel gene with thrombospondin type I domain, was expressed in the dorsal neural tube and affected Wnts mutants. Biochim. Biophys. Acta 2004; 1676:51-61. 2. Kim KA, Zhao J, Andarmani S, et al. R-spondin proteins: a novel link to beta-catenin signaling. Cell Cycle 2006; 5:23-6. 3. Parma P, Radi O, Vidal V, et al. R-spondin1 is essential in sex determination, skin differentiation and malignancy. Nat. Genet. 2006; 38:1304-9. 4. Chassot AA, Ranc F, Gregoire EP, et al. Activation of b-catenin signalling by Rspo1 controls differentiation of the mammalian ovary. Hum. Mol. Genet. 2008; 17:1278-91.

Additional Info

Recognizes human RSPO1. Species Crossreactivity: mouse and rat.