RT1B (MHC Class II Monomorphic)

The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H-2 region.\n\nApplications:\nSuitable for use in Flow Cytometry, Immunohistochemistry and Immunofluorescence. Other applications not tested. \n\nRecommended Dilutions: \nFlow Cytometry: 1:50-1:100 Use 10ul to label 10e6 cells in 100ul.\nImmunohistochemistry (Paraffin): 1:50-1:100. Antigen retrieval using heat treatment prior to staining of paraffin section is required. Sodium citrate buffer, pH 6.0 is recommended.\nImmunohistochemistry: Frozen sections\nOptimal dilution determined by the researcher. \n\nHybridoma:\nSpleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

M3887-49M

Size

100ug

Applications

FC, IF, IHC

Hosts

Mouse

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. McMaster, W. R. and Williams, A. F. (1979) Identification of Ia glycoproteins in rat thymus and purification from rat spleen. Eur. J. Immunol. 9: 426-433. 2. Fernandez, J. L. and Weeks, M. (1986) Genetic monitoring of inbred strains of mice using monoclonal antibodies to major histocompatibility haplotypes and lymphocyte alloantigens. Lab. Anim. 20: 293-297. 3. Charteris, D. G. and Lightman, S. L. (1993) In vivo lymphokine production in experimental autoimmune uveoretinitis. Immunology. 78: 387-392. 4. Whiteland, J. L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leucocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J. Histochem. Cytochem. 43: 313-320. 5. McKechnie, N. M. et al. (1997) Immunization with the cross-reactive antigens Ov39 from Onchocerca volvulus and hr44 from human retinal tissue induces ocular pathology and activates retinal microglia. J. Infect. Dis. 176: 1334-1343. 6. Male, D. K. et al. (1987) Serological evidence for a defect in RT1.B (I-A) expression by the BDIX rat strain J. Immunogenet. 14: 301-312. 7. Burrows, G. G. et al. (1998) Two-domain MHC class II molecules form stable complexes with myelin basic protein 69-89 peptide that detect and inhibit rat encephalitogenic T cells and treat experimental autoimmune encephalomyelitis. J. Immunol. 161: 5987-5996. 8. Zilka, N. et al. (2009) Human misfolded truncated tau protein promotes activation of microglia and leukocyte infiltration in the transgenic rat model of tauopathy. J. Neuroimmunology. 209: 16-25. 9. Kawamura, J. et al. (2010) Neuron-immune Interactions in the Sensitized Thalamus Induced by Mustard Oil Application to Rat Molar Pulp. J Dent Res. 89: 1309-14. 10. Calvo, M. et al. (2010) Neuregulin-ErbB signaling promotes microglial proliferation and chemotaxis contributing to microgliosis and pain after peripheral nerve injury. J. Neurosci. 30: 5437-5450. 11. McClain, J.A. et al. (2011) Adolescent binge alcohol exposure induces long-lasting partial activation of microglia. Brain Behav Immun. Jan 22. 12. Baca Jones, C.C. et al. (2009) Rat cytomegalovirus infection depletes MHC II in bone marrow derived dendritic cells. Virology. 388: 78-90.

Additional Info

Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Crossreacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region. Does not react with the rat BDIX strain due to a defect in RT1B expression. Species Crossreactivity: mouse