Rubella, gpE1, Mosaic, Recombinant (aa157-176, 374-390, 213-239) (German Measles)

Rubella virus is an enveloped positive-strand RNA virus of the family TOGAVIRIDAE: Virions are composed of three structural proteins: a capsid and two membrane-spanning glycoproteins, E2 and E1. During virus assembly, the capsid interacts with genomic RNA to form nucleocapsids. The rubella virus (RV) structural proteins: capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase.\n\nThe protein contains glycoprotein E1 immunodominant fragments, amino acids: (157-176), (374-390), (213-239).\n\nSpecificity:\nImmunoreactive with sera of Rubella Virus infected individuals.\n\nApplications:\nAntigen in ELISA and Western Blots, excellent antigen for detection of Rubella Virus with minimal specificity problems.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R9700-10

Size

100ug

Form

Supplied as a liquid in 20mM imidazole, 8M urea, 0.3M sodium chloride.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Effect of site-directed asparagine to isoleucine substitutions at the N-linked E1 glycosylation sites on rubella virus viability. Ramanujam M, Hofmann J, Atreya CD, Virus Res 2001 Dec 4;81(1-2):151-6 2. Mutations in the E1 hydrophobic domain of rubella virus impair virus infectivity but not virus assembly. Qiu Z, Yao J, Gillam S, J Virol 2000 Jul;74(14):6637-42 3. Neutralizing monoclonal antibody to the E1 glycoprotein epitope of rubella virus mediates virus arrest in VERO cells. Corboba P, Grutadauria S, Zapata M, Viral Immunol 2000;13(1):83-92 4. A single-amino-acid substitution of a tyrosine residue in the rubella virus E1 cytoplasmic domain blocks virus release. Yao J, Gillam S, J Virol 2000 Apr;74(7):3029-36 5. Mutational analysis, using a full-length rubella virus cDNA clone, of rubella virus E1 transmembrane and cytoplasmic domains required for virus release. Yao J, Gillam S, J Virol 1999 Jun;73(6):4622-30 6. Effects of mutations in the rubella virus E1 glycoprotein on E1-E2 interaction and membrane fusion activity. Yang D, Hwang D, Gillam S, J Virol 1998 Nov;72(11):8747-55.