Rubella, gpE2, aa31-105, Recombinant (German Measles)

The rubella virus (RV) structural proteins capsid, E2, and E1 are synthesized as a polyprotein precursor. The signal peptide that initiates translocation of E2 into the lumen of the endoplasmic reticulum remains attached to the carboxy terminus of the capsid protein after cleavage by signal peptidase.\n\nThe protein contains the Rubella Virus glycoprotein E2 immunodominant fragment, amino acids: (31-105).\n\nSpecificity:\nImmunoreactive with sera of Rubella Virus infected individuals.\n\nApplications:\nAntigen in ELISA and Western Blot, excellent antigen for detection of Rubella Virus with minimal specificity problems.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

R9700-06R

Size

100ug

Form

Supplied as a liquid in 20mM imidazole, 8M urea, 0.1M sodium chloride.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Rubella virus E2 signal peptide is required for perinuclear localization of capsid protein and virus assembly. Law LM, Duncan R, Nakhasi HL, J Virol 2001 Feb;75(4):1978-83 2. Effects of mutations in the rubella virus E1 glycoprotein on E1-E2 interaction and membrane fusion activity. Yang D, Hwang D, Gillam S, J Virol 1998 Nov;72(11):8747-55 3. Purification of rubella virus E1-E2 protein complexes by immunoaffinity chromatography. van Sommeren AP, Machielsen PA, Bloemers HP, J Virol Methods 1997 Jan;63(1-2):37-46 4. Evaluation of rubella virus E2 and C proteins in protection against rubella virus in a mouse model. Cusi MG, Valassina M, Wunner W, Virus Res 1995 Aug;37(3):199-208 5. Expression and characterization of secreted forms of rubella vir us E2 glycoprotein in insect cells. Seto NO, Ou D, Virology 1995 Jan 10;206(1):736-41 6. Targeting of a heterodimeric membrane protein complex to the Golgi: rubella virus E2 glycoprotein contains a transmembrane Golgi retention signal. Hobman TC, Woodward L, Mol Biol Cell 1995 Jan;6(1):7-20.