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RUNX2 (Runt-related Transcription Factor 2, Acute Myeloid Leukemia 3 Protein, AML3, CCD, CCD1, Core-binding Factor Subunit alpha-1, CBF-alpha-1, CBFA1, Oncogene AML-3, Osteoblast-specific Transcription Factor 2, OSF2, OSF-2, Polyomavirus Enhancer-binding

Cat no: R9915-12C

RUNX2 (Runt-related Transcription Factor 2, Acute Myeloid Leukemia 3 Protein, AML3, CCD, CCD1, Core-binding Factor Subunit alpha-1, CBF-alpha-1, CBFA1, Oncogene AML-3, Osteoblast-specific Transcription Factor 2, OSF2, OSF-2, Polyomavirus Enhancer-binding

RUNX2, a nuclear protein, is a member of the RUNX family of transcription factors and has an Runt DNA-binding domain. It is essential for membranous and endochondral bone formation. It not only regulates osteoblastic differentiation and skeletal morphogenesis but also acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer and with more affinity, as a subunit of a heterodimeric complex. It plays a critical role in increasing TGFBR1 expression by osteoblasts and functions in cooperation with DLX5 or a related factor to activate osteoblast-specific gene expression. Mutations lead to disorders in bone development like cleidocranial dysplasia (CCD).\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunofluorescence: 10ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R9915-12C

Size

50ug

Applications

ELISA, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

Additional Info

Recognizes human RUNX2. Species sequence homology: rat.

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