RUNX3 (Runt-related Transcription Factor 3, Acute Myeloid Leukemia 2 Protein, AML2, CBFA3, CBF-alpha-3, Core-binding Factor Subunit alpha-3, Oncogene AML-2, PEA2-alpha C, PEBP2A3, PEBP2aC, PEBP2-alpha C, Polyomavirus Enhancer-binding Protein 2 alpha C Sub

RUNX3, also called CBFA3, AML-2 or PEBP2-aC, is a member of the Runt domain family of nuclear transcriptional regulators. All of the RUNX proteins form dimers with CBF-b. The runt domain (aa54-186) is required for DNA binding, while a Pro/Ser/Thr-rich region (aa191-415) transcriptionally activates target genes. Isoform 2 has an alternate 19aa sequence in place of the N-terminal 5aa of isoform 1. The 415aa human RUNX3 shows 91% aa identity with mouse or rat RUNX3. RUNX3 is necessary for growth control of gastric epithelium, neurogenesis of dorsal root ganglia, and T cell differentiation. RUNX3 expression is frequently mutated in tumors and appears to be silenced by methylation.\n\nApplications:\nSuitable for use in Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 10ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nAA Sequence:\nMASNSIFDSFPTYSPTFIRDPSTSRRFTPPSPAFPCGGGGGKMGENSGALSAQAAVGPGGRARPEVRSMVDVLADHAGELVRTDSPNFLCSVLPSHWRCNKTLPVAFKVVALGDVPDGTVVTVMAGNDENYSAELRNASAVMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPTQVATYHRAIKVTVDGPREPRRHRQKLEDQTKPFPDRFGDLERLRMRVTPSTPSPRGSLSTTSHFSSQPQTPIQGTSELNPFSDPRQFDRSFPTLPTLTESRFPDPRMHYPGAMSAAFPYSATPSGTSISSLSVAGMPATSRFHHTYLPPPYPGAPQNQSGPFQANPSPYHLYYGTSSGSYQFSMVAGSSSGGDRSPTRMLASCTSSAASVAAGNLMNPSLGGQSDGVEADGSHSNSPTALSTPGRMDEAVWRPY\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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