RUVBL1, ID (RUVBL1, INO80H, NMP238, TIP49, TIP49A, RuvB-like 1, 49kD TATA box-binding protein-interacting protein, 54kD erythrocyte cytosolic protein, INO80 complex subunit H, Nuclear matrix protein 238, Pontin 52, TIP49a, TIP60-associated protein 54-alph

RUVBL1 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (3' to 5') activity. It is a component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL1 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1 -CTNNB1 complex. High levels of autoantibodies against RUVBL1 are detected in sera of patients with autoimmune diseases such as polymyositis/dermatomyosistis and autoimmune hepatitis.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:50-100\nImmunohistochemistry: 1:10-50\nFlow Cytometry: 1:10-50\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

041339

Size

200ul

Applications

ELISA, FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

P Type

Pab

Purity

Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.

Isotype

IgG

References

Feng, Y., et al., Cancer Res. 63(24):8726-8734 (2003).\nHawley, S.B., et al., J. Biol. Chem. 276(1):179-186 (2001).\nIkura, T., et al., Cell 102(4):463-473 (2000).\nSalzer, U., et al., Biochim. Biophys. Acta 1446(3):365-370 (1999).\nMakino, Y., et al., Biochem. Biophys. Res. Commun. 245(3):819-823 (1998).

Additional Info

Human

Alternative Names

RUVBL1, INO80H, NMP238, TIP49, TIP49A, RuvB-like 1, 49kD TATA box-binding protein-interacting protein, 54kD erythrocyte cytosolic protein, INO80 complex subunit H, Nuclear matrix protein 238, Pontin 52, TIP49a, TIP60-associated protein 54-alpha