RUVBL2 (RuvB-like 2, 48kD TATA Box-binding Protein-interacting Protein, 48kD TBP-interacting Protein, 51kD Erythrocyte Cytosolic Protein, ECP-51, INO80 Complex Subunit J, Repressing Pontin 52, Reptin 52, TIP49b, TIP60-associated Protein 54-beta, TAP54-bet

RUVBL2 is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. RUVBL2 possesses single-stranded DNA-stimulated ATPase and ATP-dependent DNA helicase (5' to 3') activity. It is a component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. RUVBL2 plays an essential role in oncogenic transformation by MYC and also modulates transcriptional activation by the LEF1/TCF1 -CTNNB1 complex. This protein may also inhibit the transcriptional activity of ATF2.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:10,000\nImmunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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