RXR alpha (RXRA, NR2B1, retinoid X receptor, alpha)

Alternate Name: RXRA, NR2B1, retinoid X receptor, alpha\n\nDomain Information: \n\nGene Ontology Terms: nucleus; regulation of transcription, DNA-dependent; retinoid-X receptor activity; signal transduction; steroid binding; steroid hormone receptor activity; transcription coactivator activity; transcription factor activity; vitamin metabolism\n\nApplication(s):\nPeptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 55kD band observed in Human epidermoid carcinoma A431 lysates (calculated MW of 50.8kD according to NP_002948). Recommended concentration: 0.5-3ug/ml.\n\n0.5ug/ml staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nPeptide Blocking:\nCorresponding peptide is available for Peptide Blocking studies. See R9985-02-P. Antibody

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SPECIFICATIONS

Catalog Number

R9985-02

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

Claudel T, Leibowitz MD, Fievet C, Tailleux A, Wagner B, Repa JJ, Torpier G, Lobaccaro JM, Paterniti JR, Mangelsdorf DJ, Heyman RA, Auwerx J. , Reduction of atherosclerosis in apolipoprotein E knockout mice by activation of the retinoid X receptor., Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2610-5. Epub 2001 Feb 20.

Additional Info

This antibody is expected to recognize an epitope corresponding to aa 14-28 of human RXR alpha protein and does not cross-react with either RXR beta or gamma. Species Crossreactivity: human, mouse, rat.