RXR gamma (Retinoid X Receptor gamma, Retinoic Acid Receptor RXR-gamma, RXRgamma, RXRG, Nuclear Receptor Subfamily 2 Group B Member 3, NR2B3, RXRC)

The human retinoid X receptors (RXRs) are encoded by three distinct genes (RXRa, RXRb, and RXRy) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid. RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors and farnesoid X receptor\n(1). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to cis-acting response elements in the promoter/enhancer region of target genes (2). RXRy expression, unlike that of RXRa and RXRb, is restricted largely to cardiac muscle, skeletal muscle, thyroid gland, and thyrotrope cells of the anterior pititary gland (3-6). It is posited that RXRy plays a pivitol role in the transcriptional control of skeletal muscle differentiation as RXRy has been\nfound to be directly associated with the promoter regions of MyoD and myogenin (7-10). RXRy is also involved in the transcriptional control of the hypothalamic-pituitary-thyroid axis through repression of the TSHb promoter (11-13).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R9990-07F

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Bov, Can, NHP

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

(1) Gronemeyer, H. et al. (2004) Nat Rev Drug Discov 3, 950-64.(6) Liu, Q. and Linney, E. (1993) Mol Endocrinol 7, 651-8. (7) Georgiades, P. and Brickell, P.M. (1997) Dev Dyn 210, 227-35. (8) Downes, M. et al. (1994) Endocrinology 134, 2658-61. (9) Muscat, G.E. et al. (1994) Nucleic Acids Res 22,583-91.\n(10) Downes, M. et al. (1993) Cell Growth Differ 4, 901-9. (11) Haugen, B.R. et al. (1997) Mol Endocrinol 11, 481-9. (12) Brown, N.S. et al. (2000) J Clin Invest 106, 73-9. (13) Sharma, V. et al. (2006) Endocrinology 147, 1438-51. (2) Mangelsdorf, D.J. et al. (1992) Genes Dev 6, 329-44. (3) Krezel, W. et al. (1996) Proc Natl Acad Sci USA 93, 9010-4. (4) Doll

Additional Info

Recognizes endogenous levels of total RXRy. Species Crossreactivity: bovine, canine, monkey, mouse, rat.

Alternative Names

OTTHUMP00000060418, Retanoic X Receptor gamma