Ryanodine Receptor 2 (RyR)

The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Flow Cytometry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1ug/ml\nImmunohistochemistry (Frozen, paraffin): 1ug/ml\nImmunocytochemistry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

R9950-10

Size

100ug

Applications

FC, ICC, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can, Ch/Bird, GP, Rabbit

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. F.A. Lai, et al.; Am. J. Physiol. 263, C365 (1992) 2. J. Junker, et al.; J. Biol. Chem. 269, 1627 (1994) 3. M.B. Bhat, et al.; Biophys. J. 77, 808 (1999) 4. F. Sedarat, et al.; Am. J. Physiol. Heart Circ. Physiol. 279, H202 (2000) 5. G.G. Du, et al.; J. Biol. Chem. 276, 31760 (2001) 6. A.N. Yusufi, et al.; Am. J. Physiol. Renal Physiol. 281, F91 (2001)

Additional Info

Recognizes canine RyR-2 at ~565kD. In non-mammalian vertebrates, a doublet is seen at 565kD representing the alpha and beta isoforms of the receptor. Crossreacts weakly with RyR-1. Species Crossreactivity: human, mouse, rat, rabbit, guinea pig, chicken, amphibian and fish.