S100 beta, CT (S100 beta, S100B, NEF, Protein S100-B, S100 Calcium-binding Protein B, S100 Calcium Binding Protein beta neural)

S100-b is a homodimeric member of the EF-hand calcium-binding protein superfamily. The protein has been implicated in cellular processes such as cell differentiation and growth, plays a role in cytoskeletal structure and function, and may have a role in neuropathological diseases, such as Alzheimers. The effects of S100-b are mediated via its interaction with target proteins (1). S100-b protein is a low molecular weight, EF-hand, Ca2(+)-binding protein widely distributed and conserved in the central nervous system of vertebrates (2). Measurements of Ca2+ binding to human brain S100-b (beta beta) protein revealed six Ca2+-binding sites and zinc-binding studies on human S100b protein showed that the protein bound two zinc ions per beta monomer. These results indicate that the Ca2+- and Zn2+-binding sites on S100-b protein are different and suggest that Zn2+ may regulate Ca2+ binding by increasing the affinity of the protein for calcium (3).\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunohistochemistry: 1:100-1:250\nImmunoprecipitation: 1:40\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

S0052-18C

Size

100ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-Glycine pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

P Type

Mab

Purity

Supernatant.

Isotype

IgG

References

1. Smith SP, et al. Structure 6(2):211-22, 1998 2. Allore RJ, et al. J Biol Chem 265(26):15537-43, 1990 3. Baudier J, et al. Biochim Biophys Acta 790(2):164-73 1984

Additional Info

Recognizes human S100 beta at ~11kD. Species Crossreactivity: mouse and rat.