S100 Calcium Binding Protein A1, Recombinant, Human (S100A1)

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.\nS100 belongs to the family of calcium binding proteins with EF-hand type Ca+2 binding motive. S100A1 (Calcium Binding Protein A1) is involved in the activation of sarcoplasmatic calcium release and the regulation of intermediate filament polymerization.\n\nActivity: \n~3nmol/mg/min using phosphatidylinositol as substrate\n\nApplications:\nSuitable for use in ELISA, Western Blot and Inhibition Assays. Other applications have not been tested. \n\nRecommended Dilutions: \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C./-70 degrees C Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0051-80H

Size

5ug

Form

Supplied as a liquid in 20mM HEPES-KOH, pH 7.0, 50mM sodium chloride, 1mM EDTA and 1mM DTT.

Purity

(same/more than) 95% (SDS-PAGE)

References

1. Co-expression/co-location of S100 proteins (S100B, S100A1 and S100A2) and protein kinase C (PKC-beta, -eta and -zeta) in a rat model of cerebral basilar artery vasospasm.\n\nNeuropathol Appl Neurobiol 2005 Dec;31(6):649-60\n\n2. S100A1 increases the gain of excitation-contraction coupling in isolated rabbit ventricular cardiomyocytes.\n\nJ Mol Cell Cardiol 2005 Dec;39(6):900-10\n\n3. The three-dimensional solution structure of Ca(2+)-bound S100A1 as determined by NMR spectroscopy.\n\nJ Mol Biol 2005 Oct 21;353(2):410-26\n\n4. S100A1 gene therapy preserves in vivo cardiac function after myocardial infarction.\n\nMol Ther 2005 Dec;12(6):1120-9\n\n5. S100A1 enhances the L-type Ca2+ current in embryonic mouse and neonatal rat ventricular cardiomyocytes.\n\nJ Biol Chem 2005 Oct 28;280(43):36019-28\n\n6. Affinity of S100A1 protein for calcium increases dramatically upon glutathionylation.\n\nFEBS J 2005 May;272(10):2557-65