S100 Calcium Binding Protein A10, Recombinant, Human (S100A10, Annexin II Ligand, ANX2L, Calpactin I Light Polypeptide, CAL1L)

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.\n\nDescription: \nThe S100 family of proteins contain two EF-hand calcium-binding motifs and are thought to be involved in the regulation of a number of cellular processes including cell cycle progression and differentiation. Recombinant Human S100A10 also called Calpactin light chain is expressed in E. coli having a molecular weight of 15.3kD fused to an amino terminal hexahistidine tag.\n\nApplications: \nRecombinant S100A10 can be used directly as a positive control in Western blotting, ELISA, immunoprecipitation and other immunological experiments. The biological activity of this product has not yet been tested.\n\nStability: \nStore at 4 degrees C if entire vial will be used within 1-2 weeks. Store, frozen at -20 degrees C for longer periods of time. Please avoid freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

S0051-87H

Size

2ug

Form

Supplied as a liquid in 1X Laemmli Buffer (25mM Tris-HCl, pH 6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue and 2.5% glycerol).

Purity

(same/more than) 95% (SDS-PAGE). 2 bands on Western Blot at 15.3 and 30.6kD, respectively representing monomeric and dimeric form.

References

1. Nomen est Omen: do antidepressants increase p11 or S100A10?\n\nJ Biomed Discov Collab 2006 Apr 13;1:5\n\n2. High expression of calcium-binding proteins, S100A10, S100A11 and CALM2 in anaplastic large cell lymphoma.\n\nBr J Haematol 2005 Dec;131(5):596-608\n\n3. Phosphatidylserine membrane domain clustering induced by annexin A2/S100A10 heterotetramer.\n\nBiochemistry 2005 Nov 22;44(46):15296-303\n\n4. Annexin A2-S100A10 heterotetramer, a novel substrate of thioredoxin.\n\nJ Biol Chem 2005 Jun 24;280(25):23584-92\n\n5. Involvement of the annexin II-S100A10 complex in the formation of E-cadherin-based adherens junctions in Madin-Darby canine kidney cells.\n\nJ Biol Chem 2005 Feb 18;280(7):6016-27\n\n6. S100A10, annexin A2, and annexin a2 heterotetramer as candidate plasminogen receptors.\n\nFront Biosci 2005 Jan 1;10:300-25