S100 Calcium Binding Protein A11, Recombinant, Human (S100A11)

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes.\n\nDescription: \nThe S100 family of proteins contain two EF-hand calcium-binding motifs and are thought to be involved in the regulation of a number of cellular processes including cell cycle progression and differentiation. This protein may function in motility, invasion and tubulin polymerisation. Recombinant Human S100A11 also called S100C is expressed in E. coli having a molecular weight of 17kD fused to an amino terminal hexahistidine tag.\n\nApplications: \nSuitable for use in Western Blot, ELISA, Immunoprecipitation and other Immunological experiments. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 2 bands at 17 and 34kD, respectively representing monomeric and dimeric form.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0051-88

Size

2ug

Form

Supplied as a liquid in PBS, 50% glycerol.

Purity

(same/more than) 95% (SDS-PAGE). 2 bands on Western Blot at 17 and 34kD, respectively representing monomeric and dimeric form.

References

1. Folding kinetics of the S100A11 protein dimer studied by time-resolved electrospray mass spectrometry and pulsed hydrogen-deuterium exchange. Biochemistry 2006 Mar 7;45(9):3005-13 2. High expression of calcium-binding proteins, S100A10, S100A11 and CALM2 in anaplastic large cell lymphoma. Br J Haematol 2005 Dec;131(5):596-608 3. Different expression of calgizzarin (S100A11) in normal colonic epithelium, adenoma and colorectal carcinoma. Int J Oncol 2006 Jan;28(1):195-200 4. Expression of S100 proteins in normal human tissues and common cancers using tissue microarrays: S100A6, S100A8, S100A9 and S100A11 are all overexpressed in common cancers. Histopathology 2005 Mar;46(3):256-69 5. Dysregulated expression of S100A11 (calgizzarin) in prostate cancer and precursor lesions. Hum Pathol 2004 Nov;35(11):1385-91 6. S100A11 gene identified by in-house cDNA microarray as an accurate predictor of lymph node metastases of gastric cancer. Oncol Rep 2004 Jun;11(6):1287-93