S100 Protein, alpha, beta

S100 protein derived from human brain tissue is a 21kD acidic calcium-binding protein synthesized by astroglial cells. It can be found predominantly as two isoforms, the alpha-beta heterodimer (S100a) and the beta-beta homodimer (S100b). The S-100 family of proteins have been implicated in a large variety of intracellular and extracellular regulatory activities, such as cellular energy metabolism, cytoskeleton modification, cell proliferation and differentiation. When glial cells are damaged, S100 protein is leaked into the extracellular matrix and cerebrospinal fluid, further releasing into the bloodstream. S100 protein can be used as a sensitive and reliable marker for nervous system damage, such as after brain damage or acute stroke. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nS0052-11A is sensitive to EDTA and other bivalent-binding agents. Improved performance can be obtained in the presence of 5mM CaCl2 in assay buffers.\nOptimal dilutions to be determined by the researcher.\n\nRecommened Pair:\nCapture: S0052-11A \nDetection: S0052-11B\n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from Balb/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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