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S100 Protein, Human

Cat no: S0052-16

S100 Protein, Human

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Antibody to S100 stains Schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. S100 is a nervous tissue specific soluble protein made in glial cells. Present in sweat glands chongrocytes and myoepithelial cells. May be found as cytoplasmic, membranous, or mixed throughout the areas of tumor. Usually cytoplasmic when present in normal tissues.\n\nS100 Protein derived from brain tissue is an acidic calcium-binding protein with the molecular weight of ~21kD. In human brain tissue S100 protein is mainly presented by two isoforms, beta-beta homodimer (S100b) and alpha-beta heterodimer (S100a).\n\nApplications: Suitable for use as a standard in immunoassay, as an immunogen for antiserum production and tracer for iodination.\n\nStorage and Stability: Lyophilized powder may be stored at -20 degrees C. Upon reconstitution, product may be stored at 4 degrees C for short-term only. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0052-16

Size

100ug

Applications

ELISA

Form

Supplied as a liquid in deionized water, 5mM 2-mercaptoethanol, 5mM EDTA.

Purity

~95% by native electrophoresis in presence of 2mM EGTA.

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