S100A8 (Calgranulin A, Calcium-binding Protein A8, CP-10, MRP8, 60B8AG, CAGA, CFAG, CGLA, L1Ag, MA387, MIF, NIF, P8)

Mouse S100A8 (also CP-10, calgranulin A and MRP8) is a 10kD member of the S100 family of calcium-binding proteins. The S100A8 protein is 89 amino acids (aa) in length and contains short, sequential modules. There is an N-terminal a-helix, followed by a calcium-binding EF-hand segment, and a C-terminal a-helix. S100A8 will noncovalently heterodimerize with S100A9. In the presence of calcium, this heterodimer will form a heterotetramer. The dimeric complex is both intracellular and extracellular. It binds heparan sulfate and is chemotactic for PMNs and macrophages. Mouse S100A8 is 80% and 57% aa identical to rat and human S100A8, respectively.\n\nApplications: \nSuitable for use in Direct ELISA, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 2ug/ml using mouse leukocyte\nImmunocytochemistry: 8-25ug/ml using immersion fixed NIH-3T3 mouse embryonic fibroblast cell line\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-74F: IgG, Murine X-Adsorbed (FITC) Pab Gt x Rt\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0052-17H1

Size

100ug

Applications

ELISA, ICC, WB

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

Additional Info

Recognizes mouse S100A8. Species Crossreactivity: recombinant human S100A8: ~40%. No crossreactivity is observed with recombinant mouse S100A4, recombinant mouse S100A9, recombinant mouse S100A10, recombinant mouse S100A11, recombinant human S100B or recombinant human S100P.

Alternative Names

S100 Calcium Binding Protein A8