Salmonella, BioAssay(TM) ELISA Kit (Qualitative)

Salmonella is a significant cause of human food-borne illness. Salmonella occurs in a wide variety of foods, however poultry meat and poultry products are recognized as a frequent source of human exposure to this pathogen. Several countries have targeted Salmonella in recent food safety initiatives. It is anticipated that limiting the contamination of food products, animal feeds and animal environments with Salmonella will reduce the economic and health impacts of food-borne infections in people. Although many methods exist for detecting Salmonella in foods, most are cumbersome, time consuming and not applicable to some production situations. Screening by the United States Biological Salmonella assay rapidly distinguishes samples that are free of Salmonella from those that are presumptively positive and require confirmatory testing.\n\nIntended Use:\nThe United States Biological Salmonella assay is an enzyme-linked immunosorbent assay (ELISA) that may be used to screen enrichment cultures of food, food products, animal feeds, environmental samples and animal and bird feces for the presence of Salmonella.\n\nTest Principle:\nTest samples are cultured as recommended and a portion of the broth culture is heated briefly to release the Salmonella antigen and to inactivate the bacteria before being added to the microwells. During the first incubation, Salmonella LPS antigen in the sample is captured by antibodies attached to the wells. In the second incubation, a peroxidase conjugated anti-Salmonella monoclonal antibody binds to the captured Salmonella antigen. After washings to remove unbound enzyme conjugate, a chromogen is added which develops a blue color in the presence of the enzyme complex and peroxide. The stop solution ends the reaction and turns the blue color to yellow.\n\nKit Components:\n1. Microtiter plate 1x96 wells each.\n2. Test strip holder: 1 (one)\n3. Negative Control: 1 vial x 2ml of a buffered base.\n4. Positive Control: 1 vial x 2ml of inactivated Salmonella LPS antigen in a buffered base.\n5. Enzyme Conjugate: 1x11ml of a monoclonal anti-Salmonella antibody conjugated to peroxidase in a buffer with preservative.\n6. Chromogen: 1x11ml of the chromogen tetramethylbenzidine (TMB).\n7. Wash concentrate solution (20x): 1 or 2x25ml of concentrated buffer and surfactant with preservative.\n8. Stop solution: 1x11ml of 1M phosphoric acid.\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for at least 6 months, For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S0060-58

Size

1Kit

Applications

ELISA

References

1. Waddell, et. al., Development of an Immunoassay for Detecting Salmonella on Chicken Carcasses. Abstract-1998 IAMFES Annual Meeting, Nashville, TN. 2. MLFP-75, In: Compendium of Analytical Methods, Health Canada. Polysciences Publications. 1995. 3. The Oxoid Manual, 20th Edition. 1990. 4. Henning and Neilsen. Journal of Immunoassay, Vol. 8, pp.297-307. 1987. 5. Wang et. al., International Journal of Food Microbiology, Vol. 29, pp. 31-41. 1996.