SAP102 (Synapse-associated Protein 102, bA291O7.3, Discs Large Drosophila Homolog 3, Discs Large Homolog 3, DLG3, KIAA1232, mKIAA1232, MRX, MRX90, Neuroendocrine dlg, NEDLG, NE-Dlg, XLMR)

Synapse-Associated Protein 102 (SAP102) is one of a family of plasma membrane-associated proteins found in synaptic junctions. Like other members of the family, SAP102 has three ~90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity.\nSAP102 is believed to participate in the clustering of certain proteins, including NMDA receptors, shaker-type potassium channels at the synaptic membrane in CNS neurons. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP102 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP102 in vitro through a pseudo-homotypic PDZ-PDZ interaction.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry (Fr). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Synaptic membrane.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

S0093-05B

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

PBS with 1 mg/ml BSA containing 0.05% sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Gomperts SN Clustering membrane proteins: It's all coming together with the PSD-95/SAP90 protein family. Cell 84:659-62 (1996). <PUBMED:8625403>Muller BM et al. SAP102, a novel postsynaptic protein that interacts with NMDA receptor complexes in vivo. Neuron 17:255-65 (1996). <PUBMED:8780649>Kornau HC et al. Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95. Science 269:1737-40 (1995). <PUBMED:7569905>

Additional Info

Species Crossreactivity: Crossreacts with Rat. Does not crossreact with Mouse (see review).