SAP102 (Synapse-associated Protein 102, bA291O7.3, Discs Large Drosophila Homolog 3, Discs Large Homolog 3, DLG3, KIAA1232, mKIAA1232, MRX, MRX90, Neuroendocrine dlg, NEDLG, NE-Dlg, XLMR)

Synapse-associated protein 102 belongs to the membrane-associated guanylate kinase protein \nfamily and is a homolog of the Drosophila dis large tumor suppressor protein. SAP102 has extensive sequence homology to the PSD 95 family of proteins that facilitate ion channel clustering at synaptic terminal (1, 2). SAP102 consists of three 90 amino acids PDZ domains at its amino terminus, a Src homolgy 3 domain and a guanylate kinase domain (3). All three PDZ domains of SAP102 participate in binding to the NMDA reception, interacting specifically with \nthe carboxy-terminal domain of the N-methyl-D- aspartate receptor 2B (NR2B). The interaction may facilitate AMPA receptor withdrawal from the postsynaptic membrane by inhibiting the ERK/MAPK pathway (3, 4). SAP102 also interacts with synaptic ras-GTPase activating protein synGAP through its PDZ domains (3). In general, the SAP102 protein is more highly expressed in non-proliferating cells than in proliferating cells, indicating a role in the negative regulation of cell growth. \n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

S0093-05C

Size

25ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. 1. Muller B.M., et al. (1996) Neuron 17: 255-265. 2. 2. Lau L. F., et al. (1996) J. Biol. Chem. 271: 21622-21628. 3. 3. Cuthbert P.C., et al. (2007) J Neuroscience 27:2673-2682. 4. 4. Lau L.F., et al. (1996) J Biol Chem 274:5782-5790.

Additional Info

Recognizes rat sap102. Species Crossreactivity: human.